Figure 2: Normalized cumulated carotenoid yield of H. pluvialis, C. zofingiensis and C. sorokiniana measured by HPLC-UV/Vis resulting from different cell disruption methods (black bar: high pressure homogenizer; light gray bar: ball mill; white bar: Ultra Turrax; dashed bar: repeated freeze and thaw; double dashed bar: freeze-drying; dotted bar: ultra-sonication; n.d.: not determined). Freeze-dried algae biomass (30, 40 and 40 mg for H. pluvialis, C. zofingiensis and C. sorokiniana) was disrupted as described, extracted via PLE (60°C, 30 min, 100 bar) with acetone:MeOH (9:1, v:v) (H. pluvialis) or DCM (C. zofingiensis, C. sorokiniana), saponified and analysed by HPLC-UV/Vis on a C30 column. Data was normalized on the highest value reached for each alga.