Figure 5: Yield of individual and total carotenoids (black bar: β-carotene; light gray bar: astaxanthin; dark gray bar: lutein; dashed bar: total carotenoids) of H. pluvialis measured by HPLC UV/Vis resulting from pressurized liquid extraction with different extraction durations: 30 mg of freeze-dried H. pluvialis biomass were disrupted using Ultra Turrax, extracted via PLE (60°C, 100 bar, DCM) for 4 different durations, saponified and analysed by HPLC -UV/Vis on a C30 column.