Cell disruption Parameters High pressure homogenizer Biomass resuspended in 10 mL of H20u, p=1000 bar, cycles n=3 Ball mill Biomass, frozen at -80°C for 1 h, 1 cycle, 50 Hz, 2 min Freeze and thaw Biomass resuspended in 10 mL of H20u, freezing -20°C, thawing at RT for 30 min, cycles n=3 Freeze-drying Biomass resuspended in 10 mL of H20ufrozen at -80°C overnight, P=0.4 mbar, overnight Ultra-sonication Biomass resuspended in 10 mL of H20u, maximum intensity, 10 min, one cycle Ultra Turrax Biomass resuspended in 10 mL of H20u, 24,000 rpm, 10 min, on ice

Table 1: Parameters for various cell disruption methods tested. Each method was performed with dried biomass of H. pluvialis (30 mg), C. zofingiensis, C. sorokiniana (40 mg).