Isoenzyme Substrate Metabolites IC50/μg(crude drug)•mL-1 CYP1A2 Phenacetin Acetaminophen <100 except WZY, BS, 2#F and 3#F, details in Table 3a (continue 1) CYP2A6 Coumarin 7-hydroxy-coumarin >100, details in Table 3b (continue 2) CYP2C19 Mephenetoin 4-hydroxy-mephenetoin <100 except WZY, BS,2#F and 3#F, details in Table 3c (continue 3) CYP2D6 Dextromethorphan Dextrorphan <100 except WZY, BS, 2#F and 3#F, details in Table 3d (continue 4) CYP3E1 chlorzoxazone 6-hydroxy-chlorzoxazone <100 except WZY, BS,2#F and 3#F, details in Table 3e (continue 5) CYP3A1/3A2 Testosterone 6β-hydroxy-testosterone <100 except WZY, BS,3#F , 4#F and 5#F, details in Table 3f (continue 6)

Note: Inhibition of rat CYP1A2 and CYP3A1/3A2 by Wuji Wan were using the single enzyme/substrate method and for others 4 CYPs was using cocktail method.

Table 2: The IC50 values of Wuji Wan on the activities of six major CYP isoenzymes in rat liver microsomes..