Wuji Wan Formulae Decomposition IC50of Formula/μg(crude drug) •mL-1 Formula IC50
A: HL B: WZY C: BS
single HLextract 28.07 ± 1.398 - - 28.07 ± 1.398
single WZY extract - 989.69 ± 110.953 - 989.69 ± 110.953
single BS extract - - 6633.28 ± 336.094 6633.28 ± 336.094
1#F extract 24.82 ± 1.851 1) 8.27 ± 0.617*) 24.82 ± 1.851Ⅲ) 57.92 ± 4.319
2#F extract 28.47 ± 2.107 18.98 ± 1.405*) 56.94 ± 4.214Ⅲ) 104.38 ± 7.725
3#F extract 45.90 ± 2.784 3) 91.79 ± 5.568*) 183.59 ± 11.136Ⅲ) 321.28 ± 19.488
4#F extract 14.85 ± 1.173 3) 2.47 ± 0.195*) 14.85 ± 1.173Ⅲ) 32.17 ± 2.541
5#F extract 24.03 ± 1.396 1) 8.01 ± 0.465*) 48.06 ± 2.791Ⅲ) 80.09 ± 4.652
6#F extract 28.59 ± 3.062 28.59 ± 3.062*) 14.29 ± 1.531Ⅲ) 71.47 ± 7.656
7#F extract 36.84 ± 2.988 2) 3.07 ± 0.249*) 36.84 ± 2.988Ⅲ) 76.76 ± 6.224
8#F extract 28.53 ± 6.143 4.75 ± 1.024*) 7.13 ± 1.536Ⅲ) 40.41 ± 8.702
9#F extract 14.72 ± 2.499 3) 7.36 ± 1.249*) 7.36 ± 1.249Ⅲ) 29.45 ± 4.997
Note: (1) the decomposition IC50 was calculated by table 1 ratios;
(2) compared with the IC50 of HL, 1)P<0.05, 2)P<0.01, 3)P<0.001; compared with the IC50 of WZY, *) P<0.001; compared with the IC50 of BS,)P<0.001.
(3) Each data point represents the mean value (± SD) of two triplicate determinations, the same below.
Table 3a: The IC50 values of Wuji Wan on the activities of CYP1A2 ( , n=6) (continue 1).