Gene |
Primer |
Primer Sequence |
Size |
| Aggrecan |
Can-Agg F |
5’ GATTGAAGTCAGTGGAGACC 3’ |
5991~ |
236 bp |
| Can-Agg R |
5’ CTGACTCATCTGTCCCTGAT 3’ |
~6226 |
| Col I a1 |
Can-C1A1 F |
5’ AACATGGAGACAGGTGAGAC 3’ |
3950~ |
235 bp |
| Can-C1A1 R |
5’ AGTGGTAGGTGATGTTCTGG 3’ |
~4184 |
| Col II a1 |
Can-C2A1 F |
5’ TACTGTTCTGAAGGATGGCT 3’ |
4313~ |
243 bp |
| Can-C2A1 R |
5’ GTGACTGGGACTGGAAAGTA 3’ |
~4555 |
| GAPDH |
Can-GAPDH F |
5’ CCAGTATGATTCTACCCACG 3’ |
135~ |
234 bp |
| Can-GAPDH R |
5’ GAAGGAGCAGAGATGATGAC 3’ |
~368 |
