Figure 4: Effects of EFs on elastic matrix synthesis by VSMCs after 21 days of culture. (A) Tropoelastin measured from spent media aliquots. (B) Matrix elastin quantified from cell layers represented in terms of alkali–soluble elastin, alkali–insoluble elastin, and total matrix elastin (soluble+insoluble). Addition of EFs resulted in a 2.6 ± 1.4 (p=0.004) fold increase in total matrix elastin, primarily in the form of soluble matrix elastin (p=0.04). ‘*’ represents significant difference from comparable control for (p<0.05).