Figure 2: Fluorescent SBA label on cryosections of generated tubules (T) kept in perfusion culture for 13 days. Culture was performed by (a) Williams’ E Medium, (b) Basal Medium Eagle (BME), (c) McCoy’s 5A Modified Medium, (d) Medium 199, (e) Iscove’s Modified Dulbecco´s Medium (IMDM), (f) Leibovitz’s L-15 Medium and (g) CO2 Independent Medium. In all of the cases, intact tubules are recognized exhibiting a lumen (arrow), an intact epithelium and a covering basal lamina (asterisk). Semithin sections of generated tubules (T) cultured in different chemically defined media for 13 days. Many vacuoles labeled by a lighted arrow head are found in series with (a’) Williams’ E Medium; (b’) Basal Medium Eagle and (c’) McCoy’s 5A Modified Medium. Few vacuoles can be detected in series with (d’) Medium 199 and (e’) Iscove’s Modified Dulbecco´s Medium. In contrast, perfect morphology without vacuoles is obtained after run with (f’) Leibovitz’s L-15 Medium and (g’) CO2 Independent Medium. PF: Polyester fibers.