Figure 3: Transmission electron microscopy of epithelial cells within regenerated tubules after 13 days of perfusion culture. In each of the cases, the luminal plasma membranes borders a lumen (arrow), while the lateral membranes are separated by an intact tight junctional complex (black arrow head). The basal membrane is in contact with a basal lamina (asterisk). A low degree of vacuoles can be seen in series with (a) Medium 199 and (b) Iscove’s Modified Dulbecco’s Medium (IMDM). In contrast, perfect morphology without arise of numerous vacuoles within the cytoplasm is registered after run with (c) Leibovitz’s L-15 Medium and (d) CO2 Independent Medium. Vacuoles are labeled by a lighted arrow head.