Figure 8: Z-axis (depth) control of DiO-labeled SMCs dispensed in agarose gel (5% buffer solution): depth separation, 1 mm; XY-separation, 1.5 mm. A: Threedimensional regionally specific dots. B: CLSM images. Valve-opening ratio, 1%; injection time, 0.1 s; concentration of cells, 1×107 cells/ml; injection pressure, 0.05 MPa.