Figure 3: Functional activity of 11β-HSD1 in intact 3D-liver microtissues over 25-days of cultivation. Five 3D-liver microtissues were combined on day seven of cultivation into one well of the assay plate (GTRAP). Microtissue spheroids were cultured in medium without dexamethasone for another 48 h. Medium was the removed and replaced by assay medium containing 50 nM radiolabeled cortisone with or without 1 µM of the selective 11β-HSD1 inhibitor T0504, followed by 24 h incubation at 37°C. Data (mean ± SD) were obtained from two independent hepatic isolations and each data point was measured in triplicate.