Figure 5: Sustained mRNA expression of Nrf2- and GR-dependent genes in 3D-liver microtissues. Freshly isolated liver cells were cultivated as 2D-sandwich monolayers or as 3Dspheroids in hanging drop cultures for three weeks at 37°C, followed by quantification of mRNA levels by real-time RT-PCR. Data (mean ± SEM) were obtained from two independent isolations, measured in triplicate, and represent ratios of ABCC3, NQO1, ORM1 and G6PD mRNA to PPIA control mRNA from 3D-spheroids or 2D-hepatocyte monolayers normalized to the values obtained after day four.