Figure 4: Morphology of bovine endothelial cells of the CPAE line seeded on PET vascular prostheses, PLG– and PLGC–impregnated PET vascular prostheses and further modified with cell-adhesive protein assemblies. Day 7 after seeding 5×104 cells/cm2. A-F: immunofluorescence of β-actin (green), cell nuclei counterstained with propidium iodide (red). G-R: staining of the cell membrane and cytoplasm with Texas Red C2-maleimide (red), cell nuclei counterstained with Hoechst #33342 (blue). Leica TCS SP2 AOBS confocal microscope (A-F), z-series of 50 images with a z-step of 1μm and Leica SPE DM 2500 (G-R), z-series of 30 images with a zstep of 1 μm, magnification 40×, zoom 2×, bar=20 μm. Polyethylene Terephthalate (PET), Poly (L-Lactide-Co-Glycolide) (PLG), Poly (L-Lactide-Co-Glycolide-Co-Ε-Caprolactone) (PLGC), Collagen type I (Co), Laminin (LM), Fibronectin (FN) and Fibrin gel (Fb).