Figure 6: Phosphorylation profiles of Akt in FN-bound (upper left), and VEGF-bound (upper right), and ERK (lower) in cultured cells. For ELISA analysis, cells were starved for 24 hours before the indicated times, and then lysed (n = 3). U0126, an inhibitor of MEK1/2 (upstream of ERK signal pathway), was added to the medium at a dose of 20 μg/ml. (Note that a separate experiment showed that ERK activation level was at similar doses). The dotted line indicates the maximum inhibition of ERK activation via the MEK1/2 pathway. Data are expressed as means ± standard deviation for 3 independent samples