Figure 8: Representative immunofluorescence images of cells cultured for up to 72 hours on VEGF-bound substrate. Collagen IV and laminin are represented by red and green, respectively (upper). FN and nuclei are represented by green and blue, respectively (lower). Deposition of collagen IV, FN, and laminin increased with time. Collagen IV and FN appear to localize at the cell-cell interfaces or in the peripheral regions of cells, whereas laminin appears to distribute homogeneously beneath the cells. Scales are 20 micrometers