Gene Primer sequence
GAPDH  
            Forward 5’-ATGACATCAAGAATGGTG-3’
            Reverse 5’-CTGTAGCCAAATTCGTTGTC-3’
Collagen type IV  
            Forward 5’-CTAATCACAAACTGAATGACTTGACTTCA-3’
            Reverse 5’-AAATGGCCCGAATGTGCTTA-3’
FN  
            Forward 5’-TGGAC CAGAGATCTTGGATGTTC-3’
            Reverse 5’-CGCCTAAA ACCATGTTCCTCA-3’
Laminin-5  
            Forward 5’-GGCTCACTCTGTATTGTTGG-3’
            Reverse 5’-ACAGAGACTGCTTTGGTGTG-3’
Table 1: Forward and reverse primers used for real-time PCR assay of ECM genes