Figure 3: Feeder-free culture of undifferentiated huESC (BJNhem 19 & 20) in conditioned medium. Undifferentiated hES cells on mitomycin-c inactivated MEF feeder-conditioned media for 4-6 days followed by formation of EBs. Details of microscopy are as follows: Photographs were taken at 40X magnification using a Leica DMIL microscope (Leica Microsystems GmbH, Germany) and a Zeiss ApoTome (Carl Zeiss Microimaging GmbH, Germany). Photographs were taken with a Zeiss Axiovert 200M microscope and Axiocam MRm and merged using Axiovision 4.6 software.