Figure 8:Expression of surface and intra-cellular markers following differentiation in induction medium SAGM in tissue culture treated plates: EB were transferred to tissue culture treated plates and grown in induction medium composed of SABM; bovine pituitary extract (BPE) 30 µg/ml, insulin 5 µg/ml, hydrocortisone (HC) 0.5 µg/ml, gentamycin sulfate-amphotericin B 0.5 µg/ml, bovine serum albumin (BSA) 0.5 mg/ml, transferrin 10 µg/ml, epinephrine 0.5 µg/ml, and recombinant human epidermal growth factor (rhEGF) 0.5 ng/ml. Cell count taken using Taliā„¢ Image based cytometer manufactured by Invitrogen, Life Technologies, USA. Photo taken by camera 1.3 megapixels, 4X objective, 4X or 16X digital zoom.