Figure 2: Differentiation assays. Cell differentiation of human adipose derived stem cells along the osteogenic (A), adipogenic (B), chondrogenic (C) and endothelial (D) pathways were detected following stainings with Alizarin Red S (A), Oil red (B) and Toluidine blue (C). The endothelial like differentiation was demonstrated by the tube formation assay (D).