| Condition |
Medium |
Serum |
Supplements |
| Control |
DMEM |
10% |
none |
| Adipogenic |
DMEM |
10% |
dexamethasone (1mM), insulin (10μg/mL), indomethacin (120mM), 3-Isobutyl-1-methylxanthine (IBMX, 0.5mM) |
| Osteogenic |
DMEM |
15% |
60 mM ascorbic acid, 10 mM β-glycerophosphate, 0.1 mM dexamethasone, |
| Chondrogenic |
DMEM |
1% |
10 ng TGF-β, 50 βM ascorbic acid and 0.5 mg/mL insulin. |
| Endothelial |
EBMTM-2 |
5% |
vascular endothelial growth factor (VEGF), human fibroblasts growth factor (hFGF), human recombinant long insulin-like growth factor-1 (R-3-IGF-1), human epidermal growth factor (hEGF), gentamycin and amphotericin (GA-1000), hydrocortisone, heparin, ascorbic acid |
|
