Figure 4: Comparison between osteogenic gene expression on MSC in Col37H and 37H. Total RNA was extracted from cells that adhered to a scaffold after the culture with Col37H and 37H in hMSC for 3, 7, and 14 days (n=9). The value of RT-PCR was calculated with adjustment for the GAPDH level in 37H and at an RQ min/max confidence level of 95% (p<0.01). Note: The expression levels of type I collagen, osteocalcin, and VEGF were significantly higher in Col37H than in 37H. An increase in gene expression levels was noted on the 3rd day of the culture.