FSC-A SSC-A CD31 (V450) CD45 (PerCP) CD146 (PE)
Negative controls MFI 31304
(27640 – 39165)		 30969
(23577 – 41964)		 3069
(1433 – 5577)		 478
(284 – 789)		 191
(103 – 319)
CV 65
(61 – 74)		 110
(90 – 125)		 358
(272 – 641)		 551
(332 –  684)		 353
(254 – 606)
Lymphocytes MFI 52296
(40618 - 62202)		 10618
(9573 – 14052)		 595
(449 – 888)		 19664
(15583 – 24714)		 160
(89 – 935)
CV 36
(28 – 39)		 21
(18 – 25)		 129
(82 – 299)		 27
(22 – 36)		 319
(123 – 586)
Endothelial cells MFI 42407
(36974 – 54995)		 52692
(38183 – 58665)		 64425
(30714 – 87822)		 1726
(1224 – 1996)		 13062
(8154 – 22679)
CV 44
(35 – 50)		 40
(36 – 47)		 51
(41 – 64)		 63
(55 – 89)		 85
(65 – 90)
P (Endothelial cells versus Lymphocytes) MFI 0.013 <0.001 <0.001 <0.001 <0.001
CV 0.001 <0.001 <0.001 <0.001 <0.001
Table 1: Forward and side scatter values and median fluorescence intensity of CD45, CD31 and CD146 expression on endothelial cells extracted mechanically from the saphenous veins of 10 patients submitted to surgery for varicose veins, as compared with that observed on residual lymphocytes present in the samples.