Figure 11: Comparison between manual and automated histomorphometry shown in Figure 8A. (A) Microscope view of DIC image with two labels (red and green) and GFP within ROI shown in Figure 8A. (B) A snap shot of signals of traditional manual analysis tracing. It shows overly smooth bone surfaces. Circled area in (A) is filled up. (C) Automated segmentation of DIC image with labels and GFP in automatic process as shown in Figure 8A. It maintains the original shape of circled area shown in (A).