Figure 3: Seeding alters gap junction intercellular communication. Cells seeded in PLGA scaffolds by micromass transferred calcenin at a higher fraction (63.2+/-10.6%) than both filtered (46.2+/-5.4%) and static seeded cells (23.6+/-6.9%). Although there was no significant differences in transfer between cells in mineralized and PLGA scaffolds (for all seeding strategies), micromass and filtered seeded cells exhibit no significant difference in transfer when seeded in a mineralized scaffold (p=9.2). Cells treated with AGA show significantly less transfer than those that were not, as well as showing no difference between seeding conditions exposed to the gap junction uncoupler. This indicates a gap junction dependent transfer of calcenin. Horizontal bars indicate groups that are not significantly different.