(A) Shortened, active follicle pre-treated with collagenase V and cultivated in medium-air-interface conditions with migrating cells on the surrounding mesh forming own colonies. (B) Non-selected cells in the adherent culture (C) Geneticine challenged cells in the adherent culture (D) differentiated ORS melanocytes (HM) (E) Control: Normal Human Epidermal Melanocytes (NHEM).
Figure 1: Current method for cultivating melanocytes from the outer root sheath cell pool of the hair follicle according to Savkovic et al. [22].