Figure 1: Investigation of cell binding to different proteins by adhesion screening on a multiple substrate array. The basic concept of a multiple substrate array screen to investigate long-term interactions of cells on either different proteins, or on serial dilutions of a given protein, or combinations thereof, is outlined in a simplified graph. Protein arrays were generated by a micro blotter on NC-coated glass slides (A). Remaining NC was blocked by StabilGuard®, and cell culture chambers were generated by a silicon frame. Cells were added in DMEM medium (B) and incubated as indicated. After removal of floating cells, cells binding to the spots on the array were stained (C) and counted by an automated process (D). Technical details can be found in the original work published recently [22].