Figure 4: Comparing the long-term binding of MSC and fibroblasts to different proteins in MSA® assays.

Binding of MSCs (n=2 donors, dark grey bars) or fibroblasts (n=2 donors, light grey bars) to extracellular matrix proteins was explored as outlined in figure 1. Binding of the cells to poly-L-lysine (PLL) served as a positive control (=100%), and binding to BSA as a negative control (=0%). Binding to (from left to right) murine type IV collagen (EHS), thrombospondin, fibronectin (from human placenta), heparan sulfate proteoglycan (HSPG), human type VI collagen (from human placenta), laminin-111 (LM111), type VI collagen, type III collagen, type I collagen, tenascin-C, vitronectin, human type V collagen, laminin-511 (LM511), and cellular fibronectin was measured. MSC bound stronger to laminin-111, type-I-, type-III-, and type-IV-collagens, whereas for other proteins (e.g. fibronectin (FN), vitronectin, laminin-511), no difference was measured.