Figure 2: Gene expression analysis of MAPK target genes and osteogenic markers. Gene expression of FGFR3 display a significant elevated expression in N540K cells stimulated with OM compared to unstimulated cells. Decreased Sox9 expression in all cell populations indicate that cells are not in the chondrogenic lineage, confirmed by Runx2 which is significantly increased in osteogenic stimulated cells carrying N540K and G380R mutations. The early osteogenic marker ALP displays a significant increased expression in all stimulated cell populations, however, the increase is over a 5-fold in the N540K mutant and a 3-fold in the G380R mutant. An increase in the mid and late osteogenic markers Coll1 and OCN can be seen in the N540K mutant.