Figure 3: Analysis on ectopic bone formation in vivo. Hematoxylin & Eosin staining display newly formed bone were cell nuclei are detected with blue colour. A-D show newly formed bone induced by cells seeded on a CopiOs™ carrier, A: Control 1, B: Control 2, C: N540K, D: G380R. E-H, display newly formed bone induced by cells seeded on CollagraftTM carrier, E: Control 1, F: Control 2, G: N540K, H: G380R. I, Show percentage of formed bone. Magnification: 100X, Scale bar: 100μm