Table 2

			Bacteriological Culture
Deer ID	Pre CCT¹	Post CCT²	Feces³	Oronasal³	Tissue⁴	Tissue Pool⁵
38	N	N	N	M. fortuitum (14, 28, 42, 56)	Neg	NA
21M	N	N	N	M. fortuitum (179) UIM (28, 42, 89)	Neg	NA
23M	N	N	N	UIM (14, 42, 89)	Neg	NA
24M	N	N	N	M. smegmatis (14) UIM (28, 42)	BCG	Thoracic, Abdominal
15	N	R	N	M. fortuitum (14) UIM (28, 42)	Neg	NA
36	N	R	N	M. fortuitum (179) UIM (28, 42, 56)	BCG	Thoracic
28M	N	R	N	UIM (14, 28, 42, 56, 89)	Neg	NA
2M	N	R	N	UIM (28, 42, 56, 89)	Neg	NA
30M	N	R	N	UIM (14, 28, 42)	Neg	NA
34M	N	R	N	BCG6 (56) UIM (28, 42, 56)	M. avium	Thoracic
38M	N	R	N	M. fortuitum (14, 179) UIM (28, 42, 56)	BCG	Thoracic
33M	N	R	N	M. fortuitum (14) UIM (28, 42, 89)	Neg	NA
3M	N	R	N	M. fortuitum (179) UIM (14, 89, 179)	Neg	NA
5M	N	R	N	UIM (14, 28, 42)	Neg	NA
7M	N	R	N	UIM (14, 28, 42, 56)	Neg	NA
22⁶	N	ND	N	M. fortuitum (14) UIM (42, 89)	BCG	Thoracic
28⁷	N	ND	N	UIM (14, 28, 42)	Neg	NA
19M⁷	N	ND	N	M. fortuitum (56) UIM (28, 42, 89)	BCG	Thoracic
18M⁷	N	ND	N	M. fortuitum (56) UIM (14, 28, 42, 89)	BCG	Thoracic, Abdomen

¹Comparative cervical tuberculin skin test prior to comingling. Neg = no evidence of prior exposure to M. bovis.
²Comparative cervical tuberculin skin test after 180 days of comingling.
³Feces and oronasal swab samples collected prior to comingling and on days 14, 28, 42, 56, 89 and 179 days of comingling. Isolate (sampling day after comingling).
⁴Isolation of mycobacteria from tissues collected at necropsy after 180 days of comingling. Neg = no mycobacteria isolated.
⁵For bacteriological isolation of mycobacteria, tissues were allocated into one of three pools; head (mandibular, parotid and medial retropharyngeal lymph nodes), thoracic
(lung, mediastinal and tracheobronchial lymph nodes), or abdominal (mesenteric and hepatic lymph nodes).
⁶Unspeciated mycobacteria later identifi ed by PCR as M. bovis BCG Danish.
⁷Deer died due to trauma unrelated to the experimental protocol prior to end of study.
UIM = Unspeciated mycobacteria found not to be M. bovis BCG Danish by PCR.
NA = not applicable.
ND = not done.

Table 2: Summary of intradermal tuberculin test and bacteriological culture results from Mycobacterium bovis BCG Danish vaccinated deer co-mingled with unvaccinated deer for 180 days.