Figure 3: GTF-I-specific IgG and IgA antibody-forming cells (AFCs) in spleen (A), and IgA AFCs in salivary glands (B). Groups of mice were nasally immunized with PBS (open bars), GTF-I alone (dotted bars), or GTF-I plus CpG ODN (closed bars) as described in the legend of Figure 1. Mononuclear cells were isolated at day 21 and, AFC responses were examined. The results are expressed as the mean ± S.E. obtained for five mice per group. The P values for IgG and IgA AFCs in spleen as well as IgA AFCs in salivary glands with GTF-I alone or with GTF-I plus CpG ODN are <0.05 when compared with PBS. *p<0.05, compared with GTF-I alone.