Figure 2: Antibody isotype generated against rTcSP2, rTcSP2-CHP, and rTcSP2-ATP in immunized mice. Antibody isotypes were determined by the ELISA method with sera obtained from mice seven days after the last immunization with recombinant proteins. Immune serum dilution was 1:500, and nonspecific adsorption to plates without antigen was measured using normal serum at the same dilution. Plotted data show optical density (OD) values for four mice per group. These data are representative of at least three independent experiments.