Figure 3: Parasitemia in mice immunized with the recombinant protein rTcSP2. BALB/c mice were immunized with PBS / TiterMax or rTcSP2/Titer-Max. Two weeks after the last immunization, four mice per group were challenged with blood trypomastigotes, and parasitemia was evaluated every three days. The values plotted are the mean ± standard deviation for four mice per group and are representative of two independent experiments. At the peak of infection, parasitemia levels were compared using one-way analysis of variance and Tukey’s honestly significant difference test. A significant difference was detected when we compared rTcSP2 to PBS (* p<0.05).