Figure 5: Percent survival of immunized and infected mice. Mice were immunized with recombinant proteins or PBS and than infected with a lethal dose of 80,000 trypomastigotes and monitored daily for 42 days. The time course of survival is shown. The values plotted are the mean ± SD for four mice per group and are representative of two independent experiments with similar results. # or * indicates a statistically significant difference with p <0.01 or p <0.05, respectively, comparing immunized to non-immunized animals by Kaplan-Meier analysis.