Figure 4: Chicken immunological responses to rCmeC vaccination (Trial 1). (A) Serum IgG response elicited by rCmeC subunit vaccination in broiler chickens. All chicken serum samples were diluted 1:100 for indirect ELISA. (B) Serum IgA and intestinal IgA (the embedded figure) levels in chickens vaccinated with rCmeC. The ELISA procedure is detailed in Materials and Methods. Each bar is the average of OD405 readings from 10 to 20 individual serum samples or 5 individual intestinal lavage samples with standard error indicated by error bar.