Plasmids or Strains Description Source or Reference Plasmids pGEMT-Easy PCR cloning vector, Ampr Promega pQE-30 Vector for N-terminal His-tagged protein construction Qiagen pQE-70 Vector for C- terminal His-tagged protein construction Qiagen pCmeC-NHIS pQE-30 ligated with cmeC segment encoding mature CmeC This study pCmeC-CHIS pQE-70 ligated with cmeC segment encoding mature CmeC This study Strains C. jejuni JL7 H49024, human isolate [29] JL10 ATCC 33291, human isolate ATCC JL12 15046764, bovine isolate [29] JL36 S3B, chicken isolate [12] JL78 W42606, human isolate [42] JL81 F34078, human isolate [42] JL83 M76297, human isolate [42] JL85 F59966, human isolate [42] JL90 M33323, human isolate [42] JL91 W11805, human isolate [42] JL93 M402, human isolate [18] JL94 E46972, human isolate [42] JL95 19094451, ovine isolate [29] JL118 CVM20088, chicken isolate [43] JL241 NCTC 11168, human isolate [44] JL242 81-176, human isolate [45] E. coli DH5α F- Φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ- Invitrogen JM109 endA1 recA1 gyrA96 thi hsdR17 (rk-, mk+) relA1 supE44 Δ(lac-proAB) [F’ traD36 lacIqZΔM15] Promega JL243 JM109 containing pCmeC-NHIS This study JL348 JM109 containing pCmeC-CHIS This study

Table 1: Key bacterial strains and plasmids used in this project.