Group No. of Chickens Day 7 Primary Immunization Day 21 Booster Immunization Sample collections C. jejuni challenge on day 35
1 20 PBS Blood: day 7,
21, 35, 42
Intestinal
lavage: day
21, 35
Yes
2 20 mLTa Yes
3 20 CmeC (50 μg) Yes
4 20 CmeC (50 μg) + mLT (10 μg) Yes
5 20 CmeC (200 μg) Yes
6 20 CmeC (200 μg) + mLT (10 μg) Yes
amLT: mucosal adjuvant LT-R192G, a mutant E. coli heat-labile toxin kindly provided by Dr. John D. Clements (Tulane University Medical Center).
Table 2: The experimental design of the first CmeC vaccination and C. jejuni challenge using broilers (Trial 1).