| Group |
No. of
Chickens |
Day 7
Primary
Immunization |
Day 21
Booster
Immunization |
Sample
collections |
C. jejuni
challenge on
day 35 |
| 1 |
20 |
PBS |
Blood: day 7,
21, 35, 42
Intestinal
lavage: day
21, 35 |
Yes |
| 2 |
20 |
mLTa |
Yes |
| 3 |
20 |
CmeC (50 μg) |
Yes |
| 4 |
20 |
CmeC (50 μg) + mLT (10 μg) |
Yes |
| 5 |
20 |
CmeC (200 μg) |
Yes |
| 6 |
20 |
CmeC (200 μg) + mLT (10 μg) |
Yes |
amLT: mucosal adjuvant LT-R192G, a mutant E. coli heat-labile toxin kindly provided by Dr. John D. Clements (Tulane University Medical Center). |
