| Group |
No. of
Chickens |
Vaccination
Route |
Day 21
Primary
Immunization |
Day 35
Booster
Immunization |
Sample
collections |
C. jejuni challenge on
day 49 |
| A |
20 |
Oral |
mLTa (70 μg) |
Blood: day 21,
35, 49, 63;
Intestinal lavage:
day 35, 49;
Cloacal swab:
day 49, 51, 53,
56, 58. |
Yes |
| B |
20 |
Oral |
CmeC (200 μg) |
Yes |
| C |
20 |
Oral |
CmeC (50 μg)+ mLT (70 μg) |
Yes |
| D |
20 |
Oral |
CmeC (200 μg) + mLT (70 μg) |
Yes |
| E |
20 |
Subcutaneous |
FIAb |
Yes |
| F |
20 |
Subcutaneous |
CmeC (200 μg) + FIAb |
Yes |
amLT: mucosal adjuvant LT-R192G, a mutant E. coli heat-labile toxin kindly provided by Dr. John D. Clements (Tulane University Medical Center). bFIA: Freund’s incomplete
adjuvant |
