Group No. of Chickens Vaccination
Route
Day 21 Primary Immunization Day 35 Booster Immunization Sample collections C. jejuni challenge on day 49
A 20 Oral mLTa (70 μg) Blood: day 21,
35, 49, 63;
Intestinal lavage:
day 35, 49;
Cloacal swab:
day 49, 51, 53,
56, 58.
Yes
B 20 Oral CmeC (200 μg) Yes
C 20 Oral CmeC (50 μg)+ mLT (70 μg) Yes
D 20 Oral CmeC (200 μg) + mLT (70 μg) Yes
E 20 Subcutaneous FIAb Yes
F 20 Subcutaneous CmeC (200 μg) + FIAb Yes
amLT: mucosal adjuvant LT-R192G, a mutant E. coli heat-labile toxin kindly provided by Dr. John D. Clements (Tulane University Medical Center). bFIA: Freund’s incomplete adjuvant
Table 3: Experimental design of the second CmeC vaccination and C. jejuni challenge using white leghorns (Trial 2).