Figure 3:a Purification of rLIC1343525-190 by Ni2+ affinity chromatography. SDS-PAGE (15%) showing lane 1- Molecular mass marker (KDa), lane 2 – Purified rLIC1343525-190 with approximately 20.2 KDa. b Circular Dichroism spectrum of rLIC1343525-190. Far UV spectra indicates that rLIC1343525-190 has regular secondary sctructure, composed mainly of a-helix.