Gene ID Primer Molecular mass (KDa) LIC10359 Forward: GGATCCGATCCTGGAAGAGATAGACTAG 20,219   Reverse: AAGCTTTTATTCCTTTTCGTTCAGAG         LIC10544 Forward: GGATCCGATTCTCAAAAATCAGATTC 51,34   Reverse: CAGCTGTTATATTTTAACCGAAATCG   LIC11211 Forward: GGATCCCAAGAACAGAGAAAATCTACG 38,846   Reverse: AAGCTTCTAATTCGTTTTAGAATCCTG         LIC10291 Forward: GGATCCGATGAGTCCGAGAAAAAAACTTATTC 17,3   Reverse: AAGCTTTTACTCTACGGTTGTAATTTC         LIC10881 Forward: CTCGAGGGCACCTTATGGGCAAAGGCTTCTC 60.574   Reverse: AAGCTTTCATTCTCTTGATTTATCGTAG   LIC13435 Forward: GGATCCCAAACTTTGGATCAGAAC 20,189   Reverse: AAGCTTTTAATTAGATGGATCTGTTTT

Table 1: Sequence of primers employed for DNA amplification and the expected molecular mass of the recombinant protein. The restriction site (HindIII, BamHI and XhoI) of each sequence is underlined.