Figure 1: Quadrant gating method. Representative dot plots are shown for the gating strategy used. A: Live cells were selected by a square gate in the forward and side scatter plot as to exclude anomalous cell particles. B: CD3+ and CD8- populations were selected. C: CD4+ populations were selected. D: Then, CD56- and CD14- populations were selected. This population number (CD3+/CD8-/CD4+/CD56-/CD14-) was used as the total CD4+ T-cell count. E: CD45RA- and CD69+ populations were selected. F: Various cytokines and other markers (such as IFN?) were plotted against CD4+ and cell population percentages were used for data analyses.