Figure 1: T cell activity in culture filtrate fractions. Figure 1 shows T cell activity a) ADA b) IFN-? c) IL-12 d) TNF-alpha in 48 hrs cell supernatants of short term PBMC induced with different phase MTB sputum culture filtrate fractions, A (3-6 day), B (10-14 day), C (21-28 day) (F-A, F-B, F-C) and BCG vaccine (F-BCG). Cell represents control without any induction. BCG vaccine is used as positive control to compare its T-cell activity with that of different culture filtrate fractions with that of MTB sputum culture. Each bar in the figure represents mean values of triplicates. P value <0.05 was considered statistically significant (* significant & ** highly significant values). (Note: we observed T-cell activity in 48 hrs culture supernatants; hence only activity in 48 hrs is reported in figure)