Figure 2: Antibody detection assay using selected panel of MTB H37RV antigens. Figure 2 shows Antibody detection assay using selected panel of MTBH37RV antigens (Ag85, CFP-10, 45kd, GroEs, ESAT-6, Hsp-16) in 72 hour cell culture supernatants of short term cultures of PBMC induced with different phase MTB sputum culture filtrate fractions, A (3-6 day), B (10- 14 day), C (21-28 day) (F-A, F-B, F-C). Each bar represents antibody titre against selected respective antigens in different culture filtrate fraction A, B, C (shown in different colors).