Post Immunization (Weeks)

Mean OD (±SD) at 450 nm

0

0.17 (±0.018)

2

0.82 (±0.03)

8

0.80 (±0.046)

12

0.86 (±0.007)
OD = Optical Density; SD = Standard Deviation
The cut-off point 0.445 demonstrates the development of anti-HIV antibodies from 2 weeks post immunization.
Table 2: Indirect ELISA detection of anti-HIV gp120 antibodies in chicken’s egg yolk