Figure 3: Evaluation of parasite load during immunotherapy. Squares represent vaccinated group (12 dogs) while circles indicates control group (5 dogs). Fine-needle biopsy were taken and Leishmania DNA copies was estimated by qPCR at each time point. The axis indicates the number of Leishmania/ml expressed in logarithmic values.
In contrast to control group the vaccinated group exhibits a reduction of parasite load since 15 days after first inoculation.