Figure 4: Immunofluorescence on BHK-21J cells transfected with RNA transcripts obtained from YF17D, panel A, B and C; pACNR-FLYF/LASV/ NP (1-876), panel D, E and F; and pACNR-FLYF/LASVNP(870-1707), panel G, H and I. Cells were stained with an antiserum against YFV NS3 (top panels A, D, and G) or an anti-FLAG epitope antibody (panel B, E and H). Bottom panel shows the results of merging the anti-YF17D NS3 and the anti-FLAG staining. The cells were fixed at 30 hr post electroporation and processed for IFA using standard protocols [45].