Recombinant YF17D/LASV-NP viruses Expected size(kDa) Estimated observed size(kDa) YF17D/LASV-VNP(1-876) 40 39 YF17D/LASV-NP(435-1296) 36 36 YF17D/LAS-NP(870-1707) 36 36 YF17D/LASV-NP(1-1296) 53 53 YF17D…3XFLAG-FMDV2A (empty vector) 5 5.6

Table 1: The predicted molecular weight of the LASV-NP-FLAG proteins and the estimated size of these proteins as detected by Western blot analysis (staining with α-FLAG antibody).