Figure 1: Cytokine expression kinetics in KdGag197-205-specific CD8+ T cells. (A) IL-3, GM-CSF, IFN-γ, TNF-α and IL-2 mRNA fold increase in KdGag197-205 peptide stimulated and unstimulated cells were evaluated at 3 hrs and 15 hrs as indicated in methods. (B-D) Two weeks following i.n./i.m. FPV-HIV/VV-HIV prime-boost immunization, splenocytes from BALB/c mice (n=4) were stimulated with KdGag197-205 peptide for 6 hrs and 20 hrs. The kinetics of (B) IFN-γ and (C) IL-3 and (D) GM-CSF protein production was determined by ICS at different time intervals (0 hr, 6 hr and 20 hr). Data represent mean values and + standard error of the mean. Data were representative of two experiments and p values were calculated using student unpaired t-test.