Strain or plasmid Relevant features References Strains     E. coli DH5α F- endA1 hsdR17 (rκ- mκ+) thi-1 λ- recA1 Ф80dlacZ∆M15 TaKaRa P. multocida     X-73 Serotype A:1 wild-type strain ATCC P-1059 Serotype A:3 wild-type strain ATCC15742 ∆hexB The hexB deletion mutant of P-1059 This study P-1059C ∆hexB complemented with plasmid pPBA1101-hexB This study Plasmids     pMD18-T Cloning vector; Ampr TaKaRA pBR322 Cloning vector，Ampr, Tetr TaKaRA pWSK29 Low-copy-number E. coli cloning vector, Ampr [24] pPBA1101 E. coli-P. multocida shuttle vector, Kanr [23] pMD18-hexABC The hexB gene and its flanking region was cloned into pMD18-T, Ampr This study pWSK29∆hexB pWSK29 containing Tetrgene, and flanking region of the hexB gene; Ampr, Tetr This study pPBA1101-hexB pPBA1101 containingthe hexB gene, Kanr This study

Table 1: Bacterial strains and plasmids used in this study.