Strain or plasmid Relevant features References
Strains    
E. coli DH5α F- endA1 hsdR17 (rκ- mκ+) thi-1 λ- recA1 Ф80dlacZ∆M15 TaKaRa
P. multocida    
X-73 Serotype A:1 wild-type strain ATCC
P-1059 Serotype A:3 wild-type strain ATCC15742
∆hexB The hexB deletion mutant of P-1059 This study
P-1059C hexB complemented with plasmid pPBA1101-hexB This study
Plasmids    
pMD18-T Cloning vector; Ampr TaKaRA
pBR322 Cloning vector,Ampr, Tetr TaKaRA
pWSK29 Low-copy-number E. coli cloning vector, Ampr [24]
pPBA1101 E. coli-P. multocida shuttle vector, Kanr [23]
pMD18-hexABC The hexB gene and its flanking region was cloned into pMD18-T, Ampr This study
pWSK29∆hexB pWSK29 containing Tetrgene, and flanking region of the hexB gene; Ampr, Tetr This study
pPBA1101-hexB pPBA1101 containingthe hexB gene, Kanr This study
Table 1: Bacterial strains and plasmids used in this study.