Figure 3: Dendritic cells from infected animals present a semimature phenotype. BALB/c (A) or C57BL/6 (B) mice were challenged with 5 or 10 metacercariae, respectively, and after three weeks from infection, splenocytes and PECs were removed, and incubated with anti-mouse CD40-FITC, CD80-PeCy5, CD86-APC and MHC-II-PE for 30 min at 4°C. Cells were analyzed by flow cytometry. Fifty thousand events were collected and gated on FSC vs. SSC dot plot, and then gated on CD11c+ dot plot. Results are expressed as mean value of quintuplicates (± SEM, indicated by error bars), each value meaning a single mouse and are representative of two different experiments. Asterisks represent differences statistically significant (*p< 0.01).